Preparation of DNA Ladder Based on Multiplex PCR Technique
نویسندگان
چکیده
منابع مشابه
Preparation of DNA Ladder Based on Multiplex PCR Technique
DNA molecular weight standard control, also called DNA marker (ladder), has been widely used in the experiments of molecular biology. In the paper, we report a method by which DNA marker was prepared based on multiple PCR technique. 100-1000 bp DNA fragments were amplified using the primers designed according to the 6631 ~ 7630 position of lambda DNA. Target DNA fragments were amplified using T...
متن کاملMethodology: simplified preparation of a DNA ladder using PCR.
Serving as a DNA molecular weight standard, the DNA ladder has been widely used in molecular biology applications. We developed a simple method for the preparation of a DNA marker, which involves designing primers to amplify 100- to 1000-bp DNA fragments using lambda DNA as a template for polymerase chain reaction, followed by extraction with phenol/chloroform, precipitation with ethanol and mi...
متن کاملConstructing a DNA ladder Range for Lambda Phage by multiplex PCR
BACKGROUND AND OBJECTIVES DNA ladder contains DNA fragments of different length but with known size, used to determine the size of unknown DNA molecules. Different DNA ladders are available for expected DNA length. Conserved sequences were selected for design of primers to generate DNA fragments of known specific size. MATERIALS AND METHODS In this study, we describe a method by which DNA lad...
متن کاملConstruction of a Synthetic Vector for Preparation of a 100 Base Pair DNA Ladder
DNA size markers are widely used to estimate the size of DNA samples on agarose or polyacrylamide gelelectrophoresis (PAGE). DNA markers can be prepared by mixing PCR products with definite sizes.Alternatively, they are prepared by restriction enzyme digestion of the genomic DNA of bacteriophages ornatural and synthetic DNA plasmids. The present study describes engineering of ...
متن کاملconstructing a dna ladder range for lambda phage by multiplex pcr
background and objectives: dna ladder contains dna fragments of different length but with known size, used to determine the size of unknown dna molecules. different dna ladders are available for expected dna length. conserved sequences were selected for design of primers to generate dna fragments of known specific size. materials and methods: in this study, we describe a method by which dna lad...
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ژورنال
عنوان ژورنال: Journal of Nucleic Acids
سال: 2010
ISSN: 2090-021X
DOI: 10.4061/2010/421803